A Digital Microfluidic Interface between Solid-phase Microextraction (spme) and Liquid Chromatography
نویسندگان
چکیده
We introduce digital microfluidic interface between solid-phase microextraction (SPME) and liquid chromatography. In the new system, a SPME fiber is used to extract analytes from a complex sample, and is then inserted into a DMF device. Solvent droplets are then used to extract analytes from the fiber with continuous actuation, followed by analysis with liquid chromatography (LC) and mass spectrometry (MS). Compared to conventional methods, the new technique allows for fast, efficient desorption of analytes from the SPME fiber using a small volume of solvent. The miniaturized system allows for preconcentration of analytes and was applied the analysis of steroid hormones in human urine.
منابع مشابه
Sample preparation in analysis of pharmaceuticals
Abbreviations: AIBN, 2,20-azo-bis-isobutyronitrile; ASE, Accelerated solvent extraction; CAR, Carboxen; DAD, Diode-array detector; DI-SPME, Direct-immersion solid-phase extraction; DSPE, Dispersive solid-phase extraction; EGDMA, Ethylene glycol dimethacrylate; ESE, Enhanced solvent extraction; ESI, Electrospray ionization; FD, Fluorescence detection; FLD, Fluorimetric detection; GC, Gas chromat...
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